mk2 sirna nucleotide  (Santa Cruz Biotechnology)

Journal: Biochemical Journal

Article Title: Phosphorylation of cAMP-specific PDE4A5 (phosphodiesterase-4A5) by MK2 (MAPKAPK2) attenuates its activation through protein kinase A phosphorylation

doi: 10.1042/bj20101184

Figure Lengend Snippet: Figure 4 Phosphorylation by MK2 attenuates the action of stimulatory phosphorylation by PKA

Article Snippet: COS1 cells were transfected using either Polyfect® reagent (Qiagen) or DEAE-dextran (Sigma–Aldrich) as described previously [10,11]. siRNAs The PolyFect® method of mammalian cell transfection (Qiagen) was used for the transfection of COS1 cells with an MK2 siRNA nucleotide from Santa Cruz Biotechnology, as reported previously [12].

Techniques: Phospho-proteomics

Journal: Biochemical Journal

Article Title: Phosphorylation of cAMP-specific PDE4A5 (phosphodiesterase-4A5) by MK2 (MAPKAPK2) attenuates its activation through protein kinase A phosphorylation

doi: 10.1042/bj20101184

Figure Lengend Snippet: Figure 5 MK2 phosphorylation of PDE4A5 alters its ability to regulate intracellular cAMP concentrations

Article Snippet: COS1 cells were transfected using either Polyfect® reagent (Qiagen) or DEAE-dextran (Sigma–Aldrich) as described previously [10,11]. siRNAs The PolyFect® method of mammalian cell transfection (Qiagen) was used for the transfection of COS1 cells with an MK2 siRNA nucleotide from Santa Cruz Biotechnology, as reported previously [12].

Techniques: Phospho-proteomics

Journal: Biochemical Journal

Article Title: Phosphorylation of cAMP-specific PDE4A5 (phosphodiesterase-4A5) by MK2 (MAPKAPK2) attenuates its activation through protein kinase A phosphorylation

doi: 10.1042/bj20101184

Figure Lengend Snippet: Figure 6 MK2 phosphorylation of PDE4A5 attenuates its ability to be reversibly recruited into intracellular aggregates by chronic rolipram treatment

Article Snippet: COS1 cells were transfected using either Polyfect® reagent (Qiagen) or DEAE-dextran (Sigma–Aldrich) as described previously [10,11]. siRNAs The PolyFect® method of mammalian cell transfection (Qiagen) was used for the transfection of COS1 cells with an MK2 siRNA nucleotide from Santa Cruz Biotechnology, as reported previously [12].

Techniques: Phospho-proteomics

Journal: Biochemical Journal

Article Title: Phosphorylation of cAMP-specific PDE4A5 (phosphodiesterase-4A5) by MK2 (MAPKAPK2) attenuates its activation through protein kinase A phosphorylation

doi: 10.1042/bj20101184

Figure Lengend Snippet: Figure 7 MK2 phosphorylation of PDE4A5 and its ability to be sequestered by partner proteins

Article Snippet: COS1 cells were transfected using either Polyfect® reagent (Qiagen) or DEAE-dextran (Sigma–Aldrich) as described previously [10,11]. siRNAs The PolyFect® method of mammalian cell transfection (Qiagen) was used for the transfection of COS1 cells with an MK2 siRNA nucleotide from Santa Cruz Biotechnology, as reported previously [12].

Techniques: Phospho-proteomics